Other

Part:BBa_K509000:Design

Designed by: Alistair Walsham   Group: iGEM11_UEA-JIC_Norwich   (2011-09-15)


Cauliflower Mosaic Virus 35S promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to produce primers to allow PCR of the target sequence to reduce the size from 1000bp to 320bp.


Source

Was supplied to the UEA-JIC team by a researcher at the University of East Anglia who supplied it in a plasmid containing the promoter, a GUS gene and a NOS terminator.

References